Effects of PR3 and protease-activated receptor-2-activators on the maturation and functions of dendritic cell-like monocytes
10.3760/cma.j.issn.1007-7480.2011.05.014
- VernacularTitle:丝氨酸蛋白酶3及蛋白酶活化受体-2激动剂对韦格纳肉芽肿病患者树突状细胞样单核细胞的影响
- Author:
Bo JIANG
;
Bingzhu HUA
;
Lingyun SUN
- Publication Type:Journal Article
- Keywords:
Wegener's granulomatosis;
Serine endopeptidases;
Receptor,protease-activated receptor-2;
Monocytes
- From:
Chinese Journal of Rheumatology
2011;15(5):341-344
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effects of protease 3(PR3)and protease-activated receptor (PAR)-2-activator on the maturation and functions of peripheral blood dendritic cell(DC)-like monocytes.Methods Density gradient centrifugation was used to isolate peripheral blood mononuclear cells(PBMC)from Wegener's granulomatosis(WG)patients and healthy controls(HC).PBMC were stimulated by LPS,human PR3,trypsin,PAR-2-agonist peptide (PAR-2-AP),LPS+PR3 or LPS+trypsin for 24 h.Flow cytometry was used to analyze the expression of PAR-2,CD80,CD83,HLA-DR on stimulated DC-like monocytes-CD14+CD16high monocytes.ELISA kit was used to test the concentration of IL-6 in the culture supernants.Mann-Whitney non-parameteric test was used fur statistical analysis.Resuits No effect of PR3,trypsin and PAR-2-AP on the expression of PAR-2,CD80,CD83,HLA-DR of DC-like monoeytes was found.LPS could significantly induce PAR-2 expression in HC[from(5.8±1.5)%to(24.5±4.5)%,P=0.002]and the expression of CD80,CD83,HLA-DR in HC and WG;PR3,trypsin,PAR-2-AP and LPS could all stimulate the secretion of IL-6.Conclusion PR3 and PAR-2 pathway-activators can not promote PAR-2expression and maturation of DC-tike monocytes,but they can induce the secretion of IL-6.
