Intracellular delivery of siRNAs mediated by ultrasound targeted microbubbles destruction and biodegradable nanoparticles carriers
10.3760/cma.j.issn.1004-4477.2011.05.024
- VernacularTitle:超声靶向微泡破碎和生物可降解纳米粒
- Author:
Qiusheng SHI
;
Ying SUN
;
Huiping ZHANG
;
Peifeng LIU
;
Jing DU
;
Fan LI
;
Lianfang DU
;
Yourong DUAN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Gene therapy
- From:
Chinese Journal of Ultrasonography
2011;20(5):445-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the intracellular delivery of siRNAs through the applications of ultrasound targeted microbubbles destruction(UTMD)and biodegradable nanoparticles carriers.Methods Preparation of nanoparticles with and without RGD sequences,parameters optimization via L16(45)orthogonal design,control experiments in groups of optimization,RGD targeted nanoparticles,non-RGD nanoparticles and blank control, and determinations by inverted fluorescence microscope and flow cytometry were performed.Results The uptake and fluorescence intensity of PC-3 cells in group of RGD targeted nanoparticle was (93.49±1.37)% and 34.28±2.06 respectively,and that in group of optimization was (88.33±1.24)% and 30.59±3.93 respectively(P＞0.05).Whereas the uptake and fluorescence intensity of PC-3 cells in group of non-RGD nanoparticles was(71.24±2.80)% and 18.39±0.90 respectively,and that in group of optimization was (84.78±2.13)% and 27.18±0.91 respectively(P＜0.05).ConclusionsThe applications of UTMD with RGD targted nanoparticles cannot increase the intracellular delivery of siRNAs.
