Expressions and correlation of CD147,MMP-9 and TIMP-1 in gliomas of different grade
10.3760/cma.j.issn.1008-6315.2011.05.005
- VernacularTitle:CD147、基质金属蛋白酶-9及其抑制物-1在不同级别胶质瘤中的表达及相关性研究
- Author:
Hongge JU
;
Shuping SHEN
;
Hong GENG
;
Liping XIE
;
Jianguo GUO
;
Jianying JIANG
;
Wei ZHANG
;
Lizhen WANG
;
Xinxin WANG
- Publication Type:Journal Article
- Keywords:
CD147;
MMP-9;
TIMP-1;
Glioma;
Immunohistochemistry;
Real time PCR
- From:
Clinical Medicine of China
2011;27(5):461-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of CD147,MMP-9 and TIMP-1 in human gliomas and analyze the correlations.Methods Expressions of CD147,MMP-9 and TIMP-1 were assessed in paraffin-embedded specimens collected from 78 gliomas and 12 benign brain lesion tissues by immunohistochemistry.Real time PCR was performed to detect CD147 mRNA expression.Results The positive rates of CD147,MMP-9 and TIMP-1 expression were 62%(48/78),71%(55/78),59%(46/78) respectively.We found a significant positive correlation between CD147,MMP-9,TIMP-1 expressions and poor gliomas differentiation by Spearman analysis(rs=0.2671-0.5631,Ps＜0.01).There was also a significant positive correlation between CD147 and MMP-9 expression(rs =0.3576,P＜0.01).In addition,the expressions of CD147(47% vs.80%,x2=9.510),MMP-9(56% vs.89%,x2=10.702),and TIMP-1(49% vs.71%,x2=4.138) were significantly higher in advanced gilomas than early gliomas(Ps＜0.05).The relative expression levels of CD147 mRNA in gliomas of Ⅰ to Ⅳ pathological grades were 0.15,0.27,0.46,0.78 respectively.Conclusion The expressions of CD147,MMP-9 and TIMP-1 were important characteristic of gliomas,which may serve as biomarkers in the glioma prognostic prediction.
