Sorting methods of breast cancer stem cells
10.3760/cma.j.issn.1673-422X.2011.05.012
- VernacularTitle:乳腺肿瘤干细胞分选的相关研究
- Author:
Chongyong GUO
;
Keying SONG
;
Ke LI
- Publication Type:Journal Article
- Keywords:
Neoplastic stem cells;
Breast neoplasms;
Drug resistance,neoplasm
- From:
Journal of International Oncology
2011;38(5):358-361
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, the theory of cancer stem cells has provided a new perspective on the treatment of cancers including breast cancer. The accurate sorting of breast cancer stem cells is critical. The sorting procedure is consist of 4 steps: isolation of side population, serum-free suspension culture,determination of specific cell surface markers and of the activity of aldehyde dehydrogenase 1 ( ALDH1 ) enzymatic through the ALDEFLUOR assay. Some studies choose drug resistance as an additional method for sorting cancer stem cells because of the enrichment of cancer stem cells after chemotherapy . The controversy about the sorting outcome of breast cancer stem cell mainly focus on molecular markers like CD44+ CD24- and ALDH1+ . The problem needed to be settled is to identify which sorting method and markers are appropriate.
