Growth inhibition and apoptosis induced by oridonin combined with valproic acid in HL-60 cells
10.3760/cma.j.issn.1673-422X.2011.05.022
- VernacularTitle:冬凌草甲素联合丙戊酸钠对HL-60细胞的生长抑制和诱导凋亡作用研究
- Author:
Huijuan ZOU
;
Guosheng JIANG
- Publication Type:Journal Article
- Keywords:
Rubescensine;
Valproic acid;
HL-60 cells;
Growth inhibitors;
Apoptosis
- From:
Journal of International Oncology
2011;38(5):390-393
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the apoptosis-inducing effect of oridonin combined with valproic acid( VPA)on leukemic cell line HL-60,and study the feasibility of oridonin combined with VPA to be used in clinical practice. Methods Oridonin of 6-12 μmol/L combined with VPA of 0. 5-1 mmol/L were added in exponential growth HL-60 cells respectively. Cell count assays were used to measure the growth inhibitory effect of oridonin combined with VPA or alone. Flow cytometry was used to evaluate apoptosis with Annexin V and propidium iodide (PI) double staining. Results Combined use of oridonin and VPA could synergistically inactivate HL-60 cells,and inhibit the cell proliferation and induce apoptosis in a dose-and time-dependent manner (P < 0.05 ). Conclusion Oridonin has a synergistic effect combined with VPA. Oridonin has a promising prospect in clinical use of leukemia.
