Effects of synthetic peptides FG loop on PC12 cells proliferation and apoptosis
10.3969/j.issn.1673-8225.2011.15.045
- VernacularTitle:人工合成多肽FGL对神经细胞模型PC12细胞增殖与凋亡的影响
- Author:
Honglong FU
;
Xuexiao MA
;
Tengbo YU
;
Bohua CHEN
;
Ning LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(15):2847-2850
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: FG loop (FGL) is a core active peptide fragment of neural cell adhesion molecule (NCAM), which can directly act on fibroblast growth factor receptor 1 (FGFR1) to activate NCAM signal pathway.OBJECTIVE: To observe the effects of synthetic peptides FGL on PC12 cells proliferation and apoptosis.METHODS: ①PC12 cells proliferation and apoptosis: The cultured PC12 cells were divided into control group and experiment group. The experimental group was added with 1% FGL peptide solution. The control group was pre-coated with poly-lysine plates. The cells were cultured 1, 3, 5, 7, 9 d respectively to detect cell proliferation by using Cell Counting Kit-8. ②PC12 apoptosis and nuclear factor kappa B mRNA detection: The PC12 cells were divided into normal group, experimental group and injury group. H2O2 was added into the injury group for 16 hours stimulation. In the experimental group, H2O2 and FGL were used for 16 hours stimulation. The cell apoptosis were detected by flow cytometry; mRNA expression of nuclear factor kappa B was detected by quantitative fluorescent polymerase chain reaction.RESULTS AND CONCLUSION: PC12 cells cocultured with FGL peptide grow well, which indicates that FGL peptides can promote PC12 cell proliferation and inhibit PC12 cell apoptosis, as well as decrease mRNA expression of nuclear factor kappa B.
