Effect of cathepsin L/G on venous vascular wall in traumatic deep vein thrombosis rat models
10.3969/j.issn.1673-8225.2011.24.039
- VernacularTitle:组织蛋白酶L/G 对创伤性深静脉血栓模型大鼠静脉壁的影响
- Author:
Wen LI
;
Jihong HU
;
Xingguo LI
;
Hongkun LI
;
Yubing ZHANG
;
Xueling ZHAO
;
Bing WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(24):4525-4529
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: At present, the basic molecular etiological mechanism and core regulatory network of deep vein thrombosis (DVT) remains uncertain, and there is not an ideal measure for early diagnosis of DVT. OBJECTIVE: To study the underlying impact of cathepsin L/G in DVT rat model. METHODS: DVT rat models (n = 50) were established by clamping both femoral vein in three different positions within 3 seconds with mosquito forceps and fixing with cast. According to different observation phases and biological situations of the femoral vein thrombosis, model rats were divided into thrombogenesis group, pre-thrombogenesis group and non-thrombogenesis group. An additional 10 normal rats served as control group. Femoral vein was obtained at corresponding time points to exact total RNA. After a gene chip-based screening, the data of gene expression were further dissected by real-time PCR. RESULTS AND CONCLUSUON: Gene chip hybridization analysis results demonstrated that differential expression of cathepsin L/G gene was significant among groups, and the expression was greatest in the thrombogenesis group, followed by pre-thrombogenesis and non-thrombogenesis groups, which was significantly greater than the control group (P < 0.05). Real-time PCR analysis results were consistent with gene chip hybridization analysis results. These indicate that DVT is associated with an increase in expression of cathepsin L/G in local venous vascular wall, and they may be candidate molecular markers for early diagnosis of deep vein thrombosis.
