Regulatory Mechanism of COX-2 by Estrogen and Progesterone in Uterine Cell Lines of Rodents.
- Author:
Byung Moon KANG
1
;
Eun Ju PARK
;
Sung Hoon KIM
;
Dae Joon CHEON
;
Hee Dong CHAE
;
Chung Hoon KIM
;
Kyun PARK
;
Yoon Seok CHANG
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
cyclooxygenase-2;
CUE-P;
CUE-V2;
estrogen;
progesterone;
expression
- MeSH:
Blotting, Western;
Cell Line*;
Cell Proliferation;
Cyclooxygenase 2;
Epithelial Cells;
Estrogens*;
Fibroblasts;
Progesterone*;
Rodentia*;
Stromal Cells
- From:Korean Journal of Obstetrics and Gynecology
2001;44(4):748-754
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To evaluate the regulatory mechanism of cyclooxygenase(COX)-2 by estrogen and progesterone in uterine cell lines of rodents MATERIALS AND METHODS: CUE-P cells, originated from the uterine endometrial epithelial cells, and CUE-V2 cells, originated from the uterine endometrial stromal cells, were incubated in the Dulbecco's modified Eagle's medium(DMEM) and F12 medium. The cellular proliferation were evaluated every 24 hours. The expression of COX-2 was measured after estrogen and progesterone supplementation by western blot. RESULTS: CUE-P cells have cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. When CUE-P and CUS-V2 cells were co-cultured with estrogen and progesterone supplementation, the expression of COX-2 was decreased after 24 hours of estrogen supplementation. The decreased expression of COX-2 was observed only when estrogen was supplemented after 9 hours of initial progesterone supplementation. CONCLUSION: The sex steroid, such as estrogen and progesterone could regulate COX-2 expression in uterine cell lines.
