Experimental study of HSP27 differential expression in left sided colon cancer and right sided colon cancer
10.3969/j.issn.1672-7347.2011.04.001
- VernacularTitle:HSP27在左侧结肠癌和右侧结肠癌差异表达的实验研究
- Author:
Haiping PEI
;
Linsheng HUANG
;
Li LIU
;
Hong ZHU
;
Liang ZENG
;
Zhiqiang XIAO
- Publication Type:Journal Article
- Keywords:
left sided colon cancer;
right sided colon cancer;
heat shock protein27;
proteomics;
immunohistochemistry
- From:
Journal of Central South University(Medical Sciences)
2011;36(4):277-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide molecular genetic basis for oncobiological difference in left sided colon cancer and right sided colon cancer. Differentially expressed proteins in left sided colon cancer and right sided colon cancer were screened by proteomic technique. Methods Tissue samples including left sided colon cancer and right sided colon cancer were collected and preserved in the -80℃ refrigerator. In the first part of our experiment, protein was separated by 2-dimensional gel electrophoresis (2-DE) and the images of the gels were acquired by the scanner and then analyzed to find the differentially expression protein-spots in different groups. The peptide mass fingerprintings (PMF) was acquired by matrix assisted laser desorptiorn/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and the proteins were identified by data searching in the Mascotdatabase. Differentially expressed proteins were assayed by RT-PCR, Western blot, and immunohistochemical method. Results Altogether 55 differentially expressed protein spots were screened and 21 spots of them were identified. Compared with the right sided colon cancer, 14 proteins were up-regulated and 7 proteins down-regulated including HSP27 in the left sided colon cancer. HSP27 expressed higher in the right sided colon cancer than in the left sided colon cancer.Conclusion There are differentially expressed proteins in left sided colon cancer and right sided colon cancer, especially difference in HSP27 expression at mRNA and protein level, which may be molecular genetic basis for oncobiological difference in left sided colon cancer and right sided colon cancer.
