Genotyping of White Spot Syndrome Virus in Chinese Cultured Shrimp during 1998-1999

Author: Yanwei TAN ; Zhengli SHI
Publication Type:Journal Article
Keywords: WSSV; Genotyping; Variable region ORF23/24; ORF14/15; ORF94,China
From: Virologica Sinica 2011;26(2):123-130
CountryChina
Language:Chinese
Abstract: Recent studies showed that white spot syndrome virus(WSSV)isolates from different geographic locations share a high genetic similarity except the variable regions in ORF23/24 and ORF14/15,and variable number of tandem repeats(VNTR)within ORF94.In this study,genotyping was performed according to these three variable regions among WSSV isolates collected during 1998/1999 from Southern China.These WSSV isolates contain a deletion of 1168,5657,5898,9316 and 11093 bp,respectively in the variable region ORF23/24compared with WSSV-TW,and a deletion of 4749 or 5622 bp in the variable region ORF14/15 relative to TH-96-II.Four types of repeat units(RUs)(6,8,9 and 13 RUs)in ORF94 were detected in these isolates,with the shortest 6 RUs as the most prevalent type.Our results provide important information for a better understanding of the spatio-temporal transmission mode and the WSSV genetic evolution lineage.