A modified isolation method for mouse intestinal intraepithelial lymphocytes
- VernacularTitle:一种小鼠肠上皮内淋巴细胞改良分离技术
- Author:
Linlin QU
;
Qiurong LI
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Intestinal intraepithelial lymphocytes;
Isolation;
Small intestine
- From:
Parenteral & Enteral Nutrition
2010;17(2):106-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore and modify the isolation method for mouse intestinal intraepithelial lymphocytes. Methods: Epithelium mucosae of mouse small intestine was incubated in iced bath and shaked in PBS containing DTT. The cell suspension was obtained after filtration with 80 and 400- screen mesh trap valve in turn. The yield, viability and purity of intestinal intraepithelial lymphocytes were observed to estimate the feasibility. Results: About (5.6±0.7)×10～6 intestinal intraepithelial lymphocytes were obtained from every 20cm samll intestine. The viabilty of intestinal intraepithelial lymphocytes was (90.46±5.71)% and the purity was (92.21±5.20)%. Conclusion: Compared with other reported isolation methods, the modifled method is convenient and esay to handling.The yield, viability and purity are high enough to be used for intestinal intraepithelial lymphocytes studies.
