Effect of soluble worm antigen and soluble egg antigen of Schistosoma japonicum on apoptosis and cell-cycle of CD4~+ T lymphocytes
- VernacularTitle:日本血吸虫可溶性成虫抗原和虫卵抗原对CD4~+T淋巴细胞凋亡和细胞周期的影响
- Author:
Cui ZHANG
;
Xiaojun CHEN
;
Jifeng ZHU
;
Ying CHI
;
Xiaoyun WEN
;
Lei HE
;
Sha ZHOU
;
Feng LIU
;
Chuan SU
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Soluble worm antigen(SWA);
Soluble egg antigen(SEA);
CD4~+T cell;
Apoptosis;
Cell-cycle
- From:
Chinese Journal of Schistosomiasis Control
2010;22(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and compare the different effects of soluble adult wornl antigen(SWA)and soluble egg antigen(SEA)of Schistosoma japonicum on the apoptosis and cell-cycle of routine CD4~+T cells.Methods Purified CD4~+T ceUs from normal C57BL/6 mice were cultured with CFSE labeled antigen presenting clls in the presence of different stimuli for 36 h.Flow cytometry(FCM)was used to detect the apoptosis of CD4~+T cells by fluorescence conjugated caspase-3 antibodie staining.The flow cytometry was used to analyze the cell-cycle of CD4~+T cells cultured as described above for 96 h by propidium iodide staining.Results Compared with the apoptosis percentage of CD4~+T cells[(1.24±0.29)%]in the SEA stimulated group,that in the SWA stimulated group[(1.52±0.38)%]did not show statistically significant difference(P>0.05).Compared with the cell percentages in G1 phase[(78.91±2.98)%],S phase[(7.39±0.85)%]and G2/M phase[(10.69±1.05)%] in the SWA stimulated group,that of the G1 phase[(59.42±1.32)%]was significantly lower,but those in the S phase[(21.07±O.88)%] and G2/M phase[(18.88±1.21)%]were significantly increased in the SEA stimulated group(P<0.01).Conclusions There is no statistically significant difference between the apoptosis levels of CD4~+T ceHs stimulated by SWA and SEA.However,SEA significantly promotes the progression of the cell-cycle of CD4~+T cells compared with SWA.
