Development of dot immunogold filtration assay kit for rapid detection of antibody to schistosome in human sera
- VernacularTitle:微量快速检测血吸虫抗体的斑点免疫金渗滤法试剂盒研制
- Author:
Shoufu JIANG
;
Qianwen QIU
;
Jing LIU
;
Xiaoping ZHANG
;
Yanyan HE
;
Xiaojiang MA
;
Li ZHANG
;
Mingmin ZHANG
;
Li CAI
- Publication Type:Journal Article
- Keywords:
Dot immunogold filtration assay (DIGFA);
Schistosomiasis;
Antibody
- From:
Chinese Journal of Schistosomiasis Control
2009;21(6):500-502
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a rapid kit applied to the field for detection of antibody to schistosome in human sera. Methods A new kit for rapid detection of antibody to schistosome was developed through improving the dot immunogold filtration assay (DIGFA). A total of 100 cases of sera from chronic schistosomiasis patients and 140 from healthy people, HBV patients and the people infected with other parasites were detected by the kit. The sensitivity, specificity, Youden's index and Kappa value were utilized as the evaluation standard. Results The sensitivity of detecting antibody to schistosome, specificity, Youden's index and Kappa value were 92% , 95.08% , 0.87 and 0.87, respectively. The cross reaction to patients with clonorchiasis was 5%. Conclusion DICFA kit is practical for antibody to schistosome detection in the field because of its advantages such as smaller serum needed and faster in reaction.
