Relationship between the fluorouracil pathway gene and effect of chemotherapy in advanced gastric cancer after surgery
10.3760/cma.j.issn.1006-9801.2011.06.012
- VernacularTitle:进展期胃癌术后化疗效果与5-氟尿嘧啶通道基因的关系
- Author:
Hua WANG
;
Xiaojiang WU
;
Zhaode BU
;
Peide DONG
;
Mingxing HOU
;
Jiafu JI
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Antineoplastic combined chemotherapy protcols;
Treatment outcome;
Molecular mechanisms of action
- From:
Cancer Research and Clinic
2011;23(6):400-402
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the fluorouracil pathway gene and effect of chemotherapy in advanced gastric cancer after surgery. Methods 52 postoperative patients with advanced gastric cancer using FOLFOX4 6-cycles combined chemotherapy were collected to set up the database. The expression of thymidine synthase (TS), dihydropyrimidine dehydrogenase (DPD) and orotate phosphoribosyltransferase(OPRT) in tumor tissue and adjacent non-tumor tissue of 52 patients with advanced gastric cancer were measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The influence of fluorouracil pathway gene on chemotherapy was investigated. Results The TS-mRNA level in tumor tissue was significantly higher than non-tumor tissue (0.92±0.28 vs 0.71±0.30) (t = 3.730, P =0.001).OPRT-mRNA level in tumor tissue was positively correlated with the non-tumor tissue (r =0.45, P =0.001). No correlations were observed among other gene expressions. Patients whose high OPRT-mRNA gene expression in their tumors and non-tumor tissue showed an obviously better survival (t = 3.036, P =0.003;t = 2.713, P =0.009). Patients with low DPD-mRNA gene expression survived longer than those with high DPD-mRNA gene expression in tumor tissue with statistical significance(t = 2.708, P =0.009), whereas prolonged survival was observed in patients with high DPD-mRNA gene expression in non-tumor tissue (t = 2.616, P =0.012).Conclusion There is close relationships between chemotherapy in advanced gastric cancer and the expression of DPD-mRNA, OPRT-mRNA;while the expression of TS-mRNA have no relation with the survival time.
