Quality evaluation of artificial musk based on its inhibitory effect on cyclooxygenase-2.
- Author:
Yun LUO
;
Cheng JIN
;
Jian ZHOU
;
Ruiqing WEN
;
Xingfeng LI
;
Ruisheng LI
;
Ming YANG
;
Xiaohe XIAO
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2011;46(4):438-42
- CountryChina
- Language:Chinese
-
Abstract:
The inhibitory ratio (1%) of artificial musk on cyclooxygenase-2 (COX-2) was determined by enzyme immunoassay (EIA). The dose-effect relationship between concentrations of artificial musk and 1% was established. It was found that artificial musk had obvious inhibitory action on COX-2. The concentration for 50% of maximum inhibitory effect (IC50) was about 2.26 mg x mL(-1). There was a good relationship between the logarithm concentrations of artificial musk and 1% when the concentrations of artificial musk ranged from 0.31-20.0 mg x mL(-1). The results indicated that this EIA method could be applied to evaluate the anti-inflammatory activity of artificial musk quickly, conveniently, sensitively and exactly. This paper provided a novel method and foundational research for the bioassay of artificial musk.
