The protective effects of SB203580 against mortality and radiation induced intestinal injury of mice.
- Author:
Jianhui CHANG
;
Heng ZHANG
;
Fangxia GUAN
;
Yueying WANG
;
Deguan LI
;
Hongying WU
;
Chengchun WANG
;
Changhui ZHOU
;
Zhibin ZHAI
;
Lu LU
;
Xiaochun WANG
;
Qi HOU
;
Aimin MENG
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2011;46(4):395-9
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the protective effects of the SB203580 against radiation induced mortality and intestinal injury of mice. A total of 67 male C57BL/6 mice (20.0-22.0 g) were matched according to body weight and randomly assigned to one of three groups: control, total body irradiation exposure (IR, 7.2 Gy) only, and IR (7.2 Gy) + SB203580 (15 mg x kg(-1)). 30 days survival rate was observed in the experiment. In intestinal injury experiment, the expression levels of caspase-3, Ki67, p53 and p-p38 were assayed in the mice intestine crypts. The results showed that the 30 days survival rate was 100% (control), 0 (IR) and 40% (IR+ SB203580), separately. Compared to the IR groups, the positive cells of caspase-3, p53 and p-p38 in crypt cells decreased 33.00%, 21.78% and 34.63%, respectively. The rate of positive cells of Ki67 increased 37.96%. Significant difference was found between all of them (P < 0.01). SB203580 potently protected against radiation-induced lethal and intestinal injury in mice, and it may be a potential radio protector.
