Expressions of anticoagulant genes KLF2 and KLF4 in blood for prediagnosing deep venous thrombosis in rats*☆

VernacularTitle:血液中抗凝基因KLF2、KLF4表达与深静脉血栓形成的预测
Author: Liqing YAO ; Yaojun DAI ; Xueling ZHAO ; Yubing ZHANG ; Ya NING ; Hongkun LI ; Wen LI
Publication Type:Journal Article
From: Chinese Journal of Tissue Engineering Research 2011;15(7):1327-1330
CountryChina
Language:Chinese
Abstract: BACKGROUND:There is lack of an effective measuring means to diagnose deep venous thrombosis (DVT) in clinic.KLF2 and KLF4 are down-expressed at prethrombotic state,which may be served as predictive molecular markers to diagnose DVT.OBJECTIVE:To explore the feasibility of KLF2 and KLF4 as molecular markers to prediagnose DVT in rats.METHODS:Totally 90 rats were obtained from 100 rats to establish traumatic DVT models and divided into the prethrombotic,thrombosis crest-time and non-thrombosis groups.The remained 10 rats served as control group.Rat blood was collected at each time point,and the expressions of KLF2 and KLF4 were detected by real-time PCR.RESULTS AND CONCLUSION:The KLF2 and KLF4 mRNA expressions in the prethrombotic group and thrombosis crest-time group were lower than that of the control group.However,the KLF2 and KLF4 mRNA expressions in the non-thrombosis group was higher than that of the control group.Therefore,KLF2 and KLF4 may be candidate molecular markers for prediagnosis of DVT in rats.