Changes of arginase Ⅰ expression in rat deep venous thrombosis models
10.3969/j.issn.1673-8225.2011.07.004
- VernacularTitle:创伤性深静脉血栓模型大鼠及精氨酸酶Ⅰ表达的变化
- Author:
En SONG
;
Yunjian LI
;
Yubing ZHANG
;
Liqing YAO
;
Rudan ZHOU
;
Hongkun LI
;
Xingguo LI
;
Chunqiang ZHANG
;
Bing WANG
;
Xueling ZHAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(7):1155-1158
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies in recent years have demonstrated that arginase Ⅰ contribute to the process of numerous cardiovascular diseases,however,most of studies focus on arteries,few regarding venous diseases.OBJECTIVE:To explore the changes of arginase Ⅰ expression in rat traumatic deep venous thrombosis models,and to analyze the possible function of arginase Ⅰ in deep venous thrombosis formation.METHODS:Totally 100 Sprague Dawley rats were randomly divided into the control and model groups.Traumatic deep venous thrombosis models were established by clamping the femoral vein and immobilizing the bilateral hind limbs (hip spica cast fixation),and assigned into initial thrombosis,peak thrombosis and non-thrombosis groups according to different observing time points and pathophysiological situations of thrombosis.Whole blood RNA of each group was extracted,and the change of arginase I expression in blood cells of each group was detected by real-time PCR.RESULTS AND CONCLUSUON:Expression of arginase Ⅰ in the peak thrombosis group was significantly increased compared with other 3 groups (P < 0.01).There were no significances among control,initial thrombosis and non-thrombosis groups (P > 0.05).The finding demonstrated that arginase Ⅰ is closely related to deep vein thrombosis formation.
