Identification of recombinant intermediates of hepatitis B virus between genotype B and C in vitro
10.3969/j.issn.1672-7347.2011.02.002
- VernacularTitle:HBV亚基因型B和C体外重组中间体的检测
- Author:
Junyi CHEN
;
Ailong HUANG
;
Li XU
;
Dianquan CHEN
;
Hong YU
;
Zhaojing ZHU
;
Zuchun HUANG
;
Zongfa YANG
;
Lishu CHEN
;
Tao TAN
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
recombinant intermediate;
genotype B;
genotype C;
in vitro
- From:
Journal of Central South University(Medical Sciences)
2011;36(2):101-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the recombinant intermediates of hepatitis B virus (HBV) between genotype B and C in vitro. Methods Vector Plenti6/V5-D-topo-X was genetically modified by genotype B and C to transfect HepG2 cells. Then the HepG2 cells were amplified and sequence of the nucleic acid after the transinfection was tested and compared with RDP3Beta40 software package and bootscanning procedure in SimPlot program package. Results Three recombinant intermediates of HBV between genotype B and C were identified in vitro. Genotype C in the precore region plus the core gene spanning nucleotide positions from 1740-1838 to 2443-2485 contributed to the recombination with genotype B. Isolate R1 recombinant intermediate had 2 break points at nt2170-2172 and nt2188-2189. Nucleic acid changed from CAC to TGT and from GA to AC, respectively. Isolate R2 recombinant intermediate had a break point at nt1740-1 838, and 3 bases changed in different nucleic acid sites: from A to T at nt1740, from C to T at nt1753, and from G to A at nt1838, respectively. Isolate R3 recombinant intermediate had a break point at nt2443-2483, and 4 bases changed in different nucleic acid sites: from C to T at nt2443, from A to G at nt2452, from T to C at nt2480, and from C to T at nt2483, respectively. Conclusion The recombinant intermediates of HBV between genotype B and C have been detected in vitro and the changes have been identified in the precore region plus the core gene in genotype B and C.
