Correlation between abnormal methylation of IGF-2 exon 9 and IGF-2 gene expression
10.3760/cma.j.issn.1673-422X.2011.04.022
- VernacularTitle:肾母细胞瘤中IGF-2外显子9甲基化状态与IGF-2表达关系的初步研究
- Author:
Huan CHENG
;
Jiantang SU
- Publication Type:Journal Article
- Keywords:
Wilms tumors;
Insulin-like growth factor Ⅱ;
DNA methylation
- From:
Journal of International Oncology
2011;38(4):315-318
- CountryChina
- Language:Chinese
-
Abstract:
Object To investigate the correlation of IGF-2 exon 9 CpG island methylation and the expression of IGF-2,and further explore the mechanism of Wilms tumor. Methods The IGF-2 exon 9 methylation status and IGF2 gene expression in 42 cases of Wilms tumor and corresponding normal tissues were detected by using methylation-sensitive restriction enzyme PCR and allele-specific IGF2 gene expression analysis.Results IGF-2 exon 9 methylation rate was 15% in W ilms tumor group, and 97% in normal tissue group. The difference was significant (P < 0.01), and the difference in pathological type was also significantly different (P < 0.01). Exon 9 unmethylated tissues showing biallelic expressions of IGF-2 were significantly higher than that of methylated tissues (P < 0.05). Conclusion IGF-2 exon 9 unmethylation may be the cause of biallelic expression of IGF, which contritues to tumorigenesis in Wilms tumor.
