Granulocyte colony-stimulating factor and interleukin-3 secretion in bone marrow stromal cells following Danggui Buxue decoction-containing serum treatment
10.3969/j.issn.1673-8225.2011.01.039
- VernacularTitle:当归补血汤载药血清干预后骨髓基质细胞中粒细胞集落刺激因子和白细胞介素3的分泌
- Author:
Tao WANG
;
Li CHEN
;
Xiaoling WANG
;
Li FENG
;
Yuhao CHEN
;
Chen TIAN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(1):171-173
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Experiment suggests that the active component of Danggui Buxue decoction, i.e. polysaccharides, can significantly promote proliferation and differentiation of hematopoietic stem cells and hematopoietic progenitor cells, and regulate hematopoietic microenvironment.OBJECTIVE: To investigate the effect of serum containing Danggui Buxue decoction on granulocyte colony-stimulating factor (G-CSF) and interleukin-3 (IL-3) secretion in mice bone marrow stromal cells (BMSCs). METHODS: BMSCs were isolated and cultured in 96-well culture plate. BMSCs morphology and growth were observed by phase contrast microscopy. The cells were divided into four groups and cultured with different concentrations of the serum containing Danggui Buxue decoction. MTT method was used to observe the proliferation of BMSCs, and ELISA method was used to observe the expression level of G-CSF and IL-3. RESULTS AND CONCLUSION: Serum containing Danggui Buxue decoction significantly promoted BMSCs proliferation, and increased the expression level of G-CSF and IL-3 secreted from BMSCs in a dose-dependent manner. Notably, serum containing equivalent dose drugs significantly promoted BMSCs proliferation, and G-CSF and IL-3 expression compared with other groups. Higher concentration weakens cell proliferation and secretion effects.
