Protective effects of IGF-1 on neurons under condition of hypoxia and the role of PI3K signal pathway

VernacularTitle:胰岛素样生长因子1对缺血缺氧神经元的保护及其与PI3K信号转导通路的关系
Author: Yibo TANG ; Wei ZHANG ; Huiling TANG ; Pengtao LI
Publication Type:Journal Article
Keywords: insulin like growth factor 1; neuron; signal pathway; oxygen and glucose deprivation model
From: Journal of Central South University(Medical Sciences) 2011;36(1):21-26
CountryChina
Language:Chinese
Abstract: Objective To investigate the protective effects of insulin like growth factor 1(IGF-1) on cortical neurons under condition of hypoxia and the possible mechanism. Methods Cerebral cortical neurons from newborn rats were cultured under the condition of oxygen and glucose deprivation (OGD) . On day 7, neurons were treated with IGF-1 or IGF-1 plus LY294002 or PD98059 under condition of OGD or normal condition. MTT assay was used to analyze the viability of neurons in each group. The expression of total Akt and p-Akt were analyzed by Western blot. Results Compared with the control, the neuron viability was significantly higher in IGF-1 treated group under normal or OGD condition (P＜0.05). The protective effects of IGF-1 were attenuated in the presence of LY294002 but not PD98059. The result of Western blot showed IGF-1 upregulated the expression of p-Akt, which was inhibited by LY294002. Conclusion PI3K pathway may play an important role in neuroprotection afforded by IGF-1.