Effects of fosinopril and losartan on renal Klotho expression and oxidative stress in spontaneously hypertensive rats
10.3969/j.issn.1672-7347.2011.01.005
- VernacularTitle:福辛普利、氯沙坦对自发性高血压大鼠Klotho表达及氧化应激的影响
- Author:
Rong TANG
;
Qiaoling ZHOU
;
Zhichun LIU
;
Zhou XIAO
;
Pouranan VEERARAGOO
- Publication Type:Journal Article
- Keywords:
fosinopril;
losartan;
Klotho;
oxidative stress;
hypertensive renal damage
- From:
Journal of Central South University(Medical Sciences)
2011;36(1):27-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore effects of fosinopril and losartan on renal Klotho expression and oxidative stress in spontaneously hypertensive rats (SHR) and the mechanisms underlying the protection against renal damage. Methods Fifteen male SHRs (22 weeks old) were randomly divided into 3 groups (n=5 in each group): a SHR group, a fosinopril group ［10 mg/(kg?d)］, and a losartan group ［50 mg/(kg?d)］. Age-matched Wistar-Kyoto (WKY) rats were chosen for a control group. Eight weeks later, tail arterial pressure, 24 hours urinary protein (Upro)，urinary N-acetyl-β-D-glucosaminidase (NAGase) were measured. Renal pathological changes were examined under light microscopy by HE staining. The renal mRNA and protein expression of Klotho were determined by RT-PCR, immunohistochemical staining or Western blot. The levels of total antioxidant capacity (TAOC), malondialdehyde (MDA), Cu/Zn superoxide dismutase (Cu/Zn-SOD), Mn superoxide dismutase (Mn-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were determined.Results The typical pathological characteristics of hypertensive renal damage were observed in the kidney of the SHR group．Compared with the SHR group, the systolic pressure, Upro, and urinary NAGase, the content of MDA and renal pathological damage was reduced while the renal Klotho expression and activities of TAOC, Cu/Zn-SOD, CAT, and GSH-Px were increased (P<0.05 or P<0.01) in the fosinopril or losartan group. There was no significant difference in renal Mn-SOD level among the 4 groups (P>0.05). Conclusion Fosinopril and losartan can exert protection against hypertensive renal damage through upregulating Klotho expression as well as reducing oxidative stress.
