Epithelial-to-mesenchymal transdifferentiation of peritoneal mesothelial cells mediated by oxidative stress in peritoneal fibrosis rats
10.3969/j.issn.1672-7347.2011.01.006
- VernacularTitle:氧化应激在腹膜纤维化大鼠模型腹膜间皮细胞转分化中的作用
- Author:
Shaobin DUAN
;
Jie YU
;
Qing LIU
;
Yuhui WANG
;
Peng PAN
;
Li XIAO
;
Guanghui LING
;
Fuyou LIU
- Publication Type:Journal Article
- Keywords:
peritoneal dialysis;
peritoneal fibrosis;
peritoneal mesothelial cell;
transdifferentiation;
oxidative stress;
probucol
- From:
Journal of Central South University(Medical Sciences)
2011;36(1):34-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of oxidative stress in the epithelial-to-mesenchymal transdifferentiation (EMT) of peritoneal mesothelial cells in rat model of peritoneal fibrosis and the effect of probucol on peritoneal fibrosis. Methods The rat model of peritoneal fibrosis was induced by 4.25% high glucose peritoneal dialysis fluid (PDF). The rats were randomly divided into 4 groups:the control group, the saline group, the peritoneal fibrosis group, and the probucol group. A 4 hour peritoneal equilibration test (PET) was performed 4 weeks later. The peritoneal function and net ultrafiltration (UF) volume were determined. The level of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in peritoneal tissue were examined. The histology of peritoneal membrane was evaluated by light microscopy. E-cadherin and α-smooth muscle actin (α-SMA) protein expression was evaluated by immunohistochemical method and Western blot.Results The mesothelial cells were detached from peritoneal membrane in peritoneal firbosis rats. Comparing with the control rats, the thickness of visceral peritoneum, the level of MDA, and the-SMA protein expression were increased while the net ultrafiltration volume, the level of GSH-Px and E-cadherin protein expression were decreased in peritoneal firbosis rats. All these changes were reversed in the rats treated with probucol.Conclusion Oxidative stress plays an important role in transdifferentiation of peritoneal mesothelial cell in the peritoneal fibrosis rats. Probucol can improve structure and function of peritoneum, and partially reverse the EMT by reducing the oxidative stress.
