Expression and cytotoxicity of a human interleukin-6 tumor necrosis factor derivative fusion protein (5＇IL6-TNF△) in vitro
- Author:
Li LIU
;
Qi XU
;
Lizhong LIU
;
Xiaomin HU
;
Baoshu XIE
;
Ying WANG
;
Min HONG
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor;
interleukin-6;
fusion protein;
antitumor
- From:
Journal of Jilin University(Medicine Edition)
1999;25(4):482-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To develop an agent that is more active against receptor-bearing target cells without increasing the toxic effect on non-target cells. Methods :By the use of molecular biology techniques,we designed and constructed a fusion protein 5＇IL6-TNF△ by connecting the human interleukin-6 (hIL-6) gene and a human tumor necrosis factor α derivative (TNF△) gene througha synthetic linker sequence followed by subsequent expression in E. Coli. Results: In cytotoxicity assay with myeloma cell line U266, the normal type of 5＇ IL6-TNF△ showed an antitumor activity 3 times higher than that of TNF△;and the antitumor activity of 5＇IL6-TNF△ blocked by IL-6Rwas only 1/30 of that of normal type of 5＇ IL6-TNF△. Meanwhile,the 5＇IL6-TNF△ blocked by an ti-TNF antibody did not show any cytotoxicity to U266 cells. In activity assay with L929 cells ,the toxic effect of the fusion protein was found 1/22 of that of TNF△. Conclusion: The 5＇IL6-TNF△fusion protein might be a useful cytotoxic agent in cancer treatment.
