Proviral Genomic Variation of EIAV Envelop gp 90 Gene Observed in Experimentally Infected Horse
- VernacularTitle:马传染性贫血病毒envgp90基因在实验感染马体内的遗传变异
- Author:
Quankai WANG
;
Hai PANG
;
Xiangang KONG
;
Hua XUAN
;
Jingang ZHANG
;
Jingliang LU
;
Enge FEI
- Publication Type:Journal Article
- Keywords:
equine infectious anemia virus;
gp90 gene;
provirus;
variation;
nucleotide sequence
- From:
Chinese Journal of Veterinary Science
1999;19(3):207-211
- CountryChina
- Language:Chinese
-
Abstract:
The present experiment was designed to observe the genetic variation of equine infectious anemia virus (EIAV) envelop gp 90 gene in infected horse. One horse was infected experimentally with P337-V70 strain and showed no clinical signs after being infected at twice with the same virus strain. Seventeen proviral sequences covering principal neutralizing domain (PND) of EIAV gp 90 gene were obtained from the buffy coat and liver of the horse through PCR amplification and cloning. Comparative analysis of the sequences revealed that some sequences contained the nucleotide insertions in the PND region. The insertions might be generated by direct repeat and strand displacement of sequence segment in their PND gene, showing different lenghts.
