Influence of rough extraction of QiZhu decoction on VEGF& KDR/flk-1 protein expression in MGC-803 cell
10.3760/cma.j.issn.1673-4246.2011.03.008
- VernacularTitle:芪竹方粗提物对MGC-803细胞VEGF、KDR/flk-1蛋白表达的影响
- Author:
Luzhou XU
;
Zhaowei SHAN
;
Wanzhou ZHAO
- Publication Type:Journal Article
- Keywords:
QiZhu decoction;
MGC-803;
VEGF;
KDR/flk-1
- From:
International Journal of Traditional Chinese Medicine
2011;33(3):214-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the influence Qizhu decoction on VEGF and its receptor KDR/flk-1 protein expression in MGC803 cell. Methods Four groups of MGC-803 Cells were established, and intervened with blank control, DMSO control, high dose Qizhu decoction, and low dose Qizhu decoction respectively. VEGF and KDR/flk-1 protein were detected by flow cytometry and western bloting. Results The value of VEGF expression was 120.0±10.8, 116.8±14.7, 95.0±12.5, and 108.4±13.5 respectively in each group. The difference between high dosage Qizhu decoction group and the bland control group was significant. Value of KDR/flk-1 were 10.4±3.5, 9.0±3.4, 6.8±2.3, and 6.8±3.5 in each group respectively. There was no significant difference among these 4 groups. The grayseale values of VEGF expression was 14.45±4.61, 12.32±3.27, 2.58±0.84, and 3.45±1.12 in each group respectively. There was significant difference between the QiZhu groups and the blank control group. Meanwhile grayseale values of KDR/flk-1 expression were 3.87±1.05, 3.55±1.32, 3.62±1.01, and 3.73±0.88 in each group respectively, showing no significant difference among 4 groups. Conclusion Rough extraction of QiZhu decoction down-regulated the protein expression of VEGF, but had no effects on the expression of KDR/flk-1.
