Determination of lobetyolin in Shengmai Granules by HPLC
10.3760/cma.j.issn.1673-4246.2011.02.015
- VernacularTitle:高效液相色谱法测定生脉颗粒中党参炔苷含量的研究
- Author:
Zhihao LI
;
Peng LI
;
Jin WU
;
Xinjian SUN
- Publication Type:Journal Article
- Keywords:
Shengmai Granules;
Lobetyolin;
HPLC
- From:
International Journal of Traditional Chinese Medicine
2011;33(2):134-136
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the determination of lobetyolin in Shengmai Granules. Methods ZORBAX SB-C18(250mm×4.6 mm,5μm) column was used, the mobile phase consisted acetonitrile:0.1% acetic acid (22:78, v/v), the flow rate was 0.8 ml/min, the column temperature was 30℃ and the detecting wavelength was 267 nm. Results The cablibration curve showed good linear relation within a range of 0.082～ 1.640 mg/ml, the average recovery was 98.5% and the RSD was 0.70%.Conclusion The method was simple, repeatable and accurate. It can be applied in quantitative determination of lobetyolin in Shengmai Granules.
