HPLC-ELSD in measuring of Astragaloside Ⅳ of Shenqi-wuweizi Tablet

VernacularTitle:参芪五味子片中黄芪甲苷的HPLC-ELSD测定方法
Author: Lianzhong ZHAO ; Haixin ZHAO ; Yuexiang SONG
Publication Type:Journal Article
Keywords: Shenqi-wuwcizitablet; HPLC; ELSD; Astragaloside
From: International Journal of Traditional Chinese Medicine 2011;33(2):137-139
CountryChina
Language:Chinese
Abstract: Objective To establish the determination method of Astragaloside Ⅳ in Shenqi-wuweizi tablet. Methods A high performance liquid chromatography method with evaporate light scattering detection was adopted. The analytes were successfully separated with Agilent-C18 column using acetonitrile and water (30:70) as mobile phase at 1.0ml/min. The parameter of ELSD was set at a evaporation tube temperature of 90℃ and nebulization at 50℃. The column temperature was set at 35℃. Results The linear range of Astragaloside Ⅳ was 41.68～521μg/ml, and the calibration curve was lnA=1.5461lnC+5.9564 with a coefficient of 0.9995 (n=6). The average recovery rate was 100.42%, with a relative standard deviation of 1.74%. Conclusion The method was simple, rapid, sensitive and accurate. Besides, it showed good repeatability as well as specificity, suitable for the quality control of Shenqi-wuweizi tablet.