Modulatory effects of nebulized nitric oxide donor on inflammatory response in newborn piglets with acute respiratory distress syndrome
10.3760/cma.j.issn.1673-4912.2011.01.018
- VernacularTitle:雾化吸入NO供体对新生猪急性呼吸窘迫综合征炎症反应的干预作用
- Author:
Shaohua WANG
;
Bing HUANG
;
Yongming WANG
;
Hongxing DANG
;
Feng XU
;
Fengwu KUANG
- Publication Type:Journal Article
- Keywords:
Acute respiratory distress syndrome;
Neb-SNP;
Nuclear factor-kappa B;
Cytokine
- From:
Chinese Pediatric Emergency Medicine
2011;18(1):53-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate modulatory effects of Neb-SNP on inflammatory response and to explore the protection mechanisms of Neb-SNP in newborn piglets with ARDS. Methods Forty-five neonatal swines were randomly divided into five groups:group A (controlled group ,n = 9), group B (physiological saline group,n =9),group C (Neb-SNP 1 mg/ml,0. 9% NaCl, n = 9), group D (Neb-SNP 5 mg/ml,0. 9% NaCl, n = 9) and group E (Neb-SNP 10 mg/ml,0. 9% NaCl, n = 9). The pathological changes and activity of NF-κB in the lung tissue ,TNF-α ,IL-10 and IL-12 concentrations in serum at 30 minutes,60 minutes and 120 minutes after aerosol inhalation were observed. Results Activity of NF-κB and serum concentrations of TNF-α and IL-12 in the Neb-SNP treated group were lower than group B(P ＜0. 05) ,and serum IL-l0 concentration was obviously higher in the Neb-SNP group(P ＜0. 05). With an increase of Neb-SNP concentration,activity of NF-κB and serum concentrations of TNF-α and IL-12 were obviously increased, while serum concentrations of IL-10 was increased in group D and group E than that of group C (P ＜ 0. 05).Conclusion Inhalation of Neb-SNP reduced lung injury induced ARDS through lowering NF-κB activity and inhibiting expression of harmful inflammatory cytokines.
