Contortrostatin on the inhibition of a human malignant glioma cell line U87 line in vivo
- VernacularTitle:蛇毒解聚素抑制荷U87胶质瘤裸鼠移植瘤的实验研究
- Author:
Shaojian ZENG
;
Hang SHU
;
Guangzhong CHEN
;
Zhoojie LI
;
Shengquan ZHAN
;
Xiaofeng LIN
;
Dong ZHOU
- Publication Type:Journal Article
- Keywords:
Contortrostatin;
Glioma;
Nude mice
- From:
Chinese Journal of Postgraduates of Medicine
2009;32(z1):3-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of contortrostafin on human glioma xengoraft model and explore its mechanism.Method Animal models were established on BALB/c nude mice with subcutaneously transplanted neoplasma of U87 glioma.The nude mice bearing withU87 slioma were devided randomly into two groups,which were treated without drugs or with doses of 40 μg/d.The dimension of xenografts was measured and the living state of nude mice with U87 glioma was observed.The protein expression of microvesel density(MVD),bFGF in each paraff in imbedded xenograft of nude mice bearing with U87 glioma were detected by immunohistochemistry staining.Result Contortrostafin inhibited the growth of xenografts in nude mice with U87 glioma(P＜0.05),and the tumor volume was reduced by 50%.Meanwhile the control group highly expressed bFGF at the level of protein,coupling with increase of MVD.Compared with the dissolvent control group,contortrostatin treated group showed lower expression of bFGF,(P＜0.01)and more decrease in MVD(P＜0.01).Conclusion Contortrostatin diffusa has significant inhibitive effect on human glioma xenograft.
