Quantitative detection of mutation of K-ras gene at codon 12
10.3760/cma.j.issn.1674-1935.2011.01.011
- VernacularTitle:胰腺癌组织K-ras基因第12密码子突变的定量检测
- Author:
Junjun GU
;
Jun GAO
;
Yanfang GONG
;
Haojie HUANG
;
Xiaowei WANG
;
Hua LU
;
Zhaoshen LI
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
K-ras gene;
Rreal-time PCR;
Mutation
- From:
Chinese Journal of Pancreatology
2011;11(1):31-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To quantitatively analyze the K-ras gene mutation at codon 12 in pancreatic cancer tissues and the relationship between K-ras gene mutation and clinicopathological parameters. Methods Quantitative detection of K-ras gene at codon 12 in 93 pairs of pancreatic cancer and adjacent tissues were performed by using PNA-mediated PCR clamping with two different fluorescence labeled probes. The quantity of mutation was expressed by percentage of mutation. The percentage of K-ras gene mutation = the copy of K-ras mutation/(copy of wild type K-ras + copy of K-ras mutation) × 100%. Results The percentage of mutation of K-ras gene at codon 12 in pancreatic cancer and adjacent tissues were 83.9% and 65.6%, and the difference was statistically significant(P ＜ 0. 05); and the quantity of mutation were (13.385 ± 1. 745) % and (2. 246 ±0. 728) %, and the difference was also statistically significant(P ＜ 0. 05). The quantity of mutation of K-ras gene at codon 12 was not associated with clinicopathological parameters. Conclusions The percentage of K-ras gene mutation, as well as the quantity of K-ras gene mutation was different in pancreatic carcinoma and adjacent tissues.
