Malignant phenotype regulated by endocrine glands derived VEGF in pancreatic cancer cells MiaPaCaⅡ
10.3760/cma.j.issn.1674-1935.2011.02.007
- VernacularTitle:内分泌腺来源的血管内皮生长因子对胰腺癌MiaPaCa Ⅱ细胞恶性表型的调控作用
- Author:
Xiaozhong GUO
;
Linan REN
;
Xu LIU
;
Feng LIU
;
Deli ZOU
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Vascular endothelial growth factor;
Phenotype
- From:
Chinese Journal of Pancreatology
2011;11(2):98-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the endocrine glands derived VEGF (EG-VEGF) influence on growth, migration and apoptosis of pancreatic cancer cells MiaPaCa Ⅱ. Methods MiaPaCa Ⅱ were treated by 100,200 ng/ml EG-VEGF for 24, 48, 72, 96 h, and MTT assay was used to determine the proliferation; and cell scratch experiment was used to investigate the percentage of cell migration distance, flow cytometry was used to measure the apoptosis of the cancer cells. Results After MiaPaCa Ⅱ cells were treated by 0, 100,200 ng/ml EG-VEGF for 72 h, the proliferation of MiaPaCa Ⅱ was 0. 253 ± 0. 012 , 0. 374 ± 0.013,0. 383 ±0.015, respectively EG-VEGF could significantly promote the proliferation of MiaPaCa Ⅱ ( P ＜ 0. 05 ). After MiaPaCa Ⅱ cells were treated by 0, 100 ng/ml EG-VEGF for 24 h, the percentage of cell migration distance was (27.40 ± 3.45 ) % and ( 13.21 ±4.65 ) % ,respectively with statistical difference ( P ＜ 0.05 ), EG-VEGF could significantly promote the migration ability of MiaPaCa Ⅱ cells and inhibite the apoptosis. Conclusions After EG-VEGF treatment, the growth and migration ability of MiaPaCa Ⅱ cells increases, apoptosis decreases.
