Effect of Oxidative Stress on the Expression of Gelatinases in Cultured RPE.
- Author:
Young Chul PARK
1
;
Gwang Ju CHOI
Author Information
1. Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Korea. gjchoi@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Antioxidant;
Matrix metalloproteinase;
Oxidative stress;
Retinal pigment epithelial cell
- MeSH:
Acetylcysteine;
Antioxidants;
Blotting, Western;
Culture Media, Conditioned;
Epithelial Cells;
Gelatinases*;
Humans;
Matrix Metalloproteinase 2;
Matrix Metalloproteinase 9;
Oxidative Stress*;
Paraquat;
Retinaldehyde;
Reverse Transcriptase Polymerase Chain Reaction;
RNA, Messenger
- From:Journal of the Korean Ophthalmological Society
2005;46(11):1903-1910
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the expression pattern of gelatinases in the human retinal pigment epithelial cell line ARPE-19 in response to oxidative stress. METHODS: ARPE-19 cells were exposed to paraquat for 72 hours. The cells were assayed for the mRNA expression of gelatinase A and gelatinase B by reverse transcriptase polymerase chain reaction (RT-PCR). The conditioned media were assayed for the production of gelatinases protein by Western blotting. Also, the inhibitory effect of the antioxidants against oxidative stress to the cells was evaluated. RESULTS: The gelatinase levels in the RPE cells increased under oxidative stress compaired with the control (p<0.05). Also, the levels decreased when the cells were incubated with the antioxidant N-acetylcysteine (NAC). CONCLUSIONS: Oxidative stress upregulates gelatinase expression in ARPE-19 cells.
