Effect of Triamcinolone Acetonide on the Survival and Nitric Oxide Production in Cultured Human Tenon's Capsule Fibroblasts.
- Author:
Hyo Won LEE
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Fibroblasts;
Nitric oxide;
Triamcinolone acetonide;
Wound healing
- MeSH:
Cell Survival;
Dexamethasone;
Fibroblasts*;
Humans*;
Indomethacin;
Nitric Oxide*;
Tenon Capsule*;
Triamcinolone Acetonide*;
Triamcinolone*;
Wound Healing
- From:Journal of the Korean Ophthalmological Society
2005;46(11):1919-1924
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effect of triamcinolone acetonide (TA) on cell survival and nitric oxide (NO) production in cultured human Tenon's capsule fibroblast (FB) cells. METHODS: After exposed to TA, indomethacin, and dexamethasone for 2 days, cell survival and nitrite production of primarily cultured human FB cells were assessed with MTT and Griess assays, respectively. RESULTS: Cell survival increased after exposure to TA at low concentrations but decreased at high concentrations. TA decreased the production of NO significantly (p<0.05). Exposure to indomethacin and dexamethasone revealed similar results. CONCLUSIONS: Both the biphasic effect on cell survival and the decreased production of NO induced by TA in FB cells could affect conjunctival wound healing.
