Expression and clinical significance of osteopontin and MMP-9 in pancreatic carcinoma
10.3760/cma.j.issn.1008-1372.2011.03.009
- VernacularTitle:骨桥蛋白和基质金属蛋白酶-9在胰腺癌中的表达及其临床意义
- Author:
Xiping CHEN
;
Lingxin MENG
;
Aili ZHANG
;
Hong ZHANG
;
Jiangtao LI
;
Shuyan SUN
- Publication Type:Journal Article
- Keywords:
Osteopontin/ME;
Matrix metalloproteinase 9/ME;
Pancreatic neoplasms/ME
- From:
Journal of Chinese Physician
2011;13(3):312-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and the role of osteopontin(OPN)and matrix metalloproteinase-9(MMP-9)in the invasion and metastasis of pancreatic carcinoma.Methods Human pancreatic cancer cell line MIA PaCa-2 was cultured in vitro.The location of OPN protein in pancreatic tumor cell line was detected by immunofluorescence staining.100 cases of pancreatic cancer and 40 cases of adjacent normal pancreatic tissues were used to analysis.The expressions of OPN and MMP-9 were investigated by immunohistochemistry assay.Results OPN expressed in the cell membrane and cytoplasm,OPN and MMP-9 in pancreatic cancer tissues positive rates were 73.0%(73/100)and 68.0%(68/100),which were higher than that in adjacent normal pancreatic tissues(all P ＜ 0.05).OPN and MMP-9 expression were associated with pancreatic tumor grade,lymph node metastasis and perineural invasion of pancreatic cancer(P ＜ 0.05).OPN and MMP-9 in pancreatic cancer tissues were positively correlated(r,=0.39,P ＜ 0.01).Conclusions OPN and M MP-2 might play an important role in the development of invasion and metastasis of pancreatic carcinoma.OPN and MMP-2 might play synergetic roles in the progression of pancreatic carcinoma.
