TRPC6 mediates the proliferation of human umbilical vein endothelial cells induced by DU145 conditioned medium

VernacularTitle:TRPC6介导DU145细胞条件培养液诱导人脐静脉内皮细胞增殖的研究
Author: Wei QU ; Gang SUN ; Yong WANG ; Yili LIU ; Ping WANG
Publication Type:Journal Article
Keywords: Transient receptor potential channels/ME; Prostatic neoplasms; Tumor cells,cultured; Endothelial cells; Umbilical veins; Cell proliferation
From: Journal of Chinese Physician 2011;13(1):12-15
CountryChina
Language:Chinese
Abstract: Objective To investigate the influence of transient receptor potential canonical 6 (TRPC6) on the proliferation of hUVEC induced by the supematant from human prostate cancer cells (DU145). Methods Experiment group was treated with the mixture of DU145 cells supernatant and DMEM medium in different ratio, control group was treated with DMEM medium. The change of cell proliferation was detected by MTT method, and TRPC6 gene and protein was detected by PCR and Western-blot methods respectively. Results After incubated for 48h with the supematant from cultured DU145 cells,the population of hUVECs increased obviously, and the expression of TRPC6 genes and protein had clearly up-regulation. Conclusion The supernatant from cultured DU145 cells could effectively enhance the proliferation of hUVECs. The mechanisms might be that the proliferation of human umbilical vein endothelial cells was mediated by TRPC6.