Transplantation of NGF modified umbilical cord mesenchymal stem cells improves neurological deficits in rats subjected to brain trauma

VernacularTitle:人脐带干细胞携带NGF基因脑内移植对脑损伤大鼠神经行为学的影响
Author: Yuan YUAN ; Qiqin DAN ; Jia LIU
Publication Type:Journal Article
Keywords: Contusion brain injury; Umbilical cord mesenchymal stem cells (UCMSC); Neural growth factor; Neurological function; Rat
From: Chinese Journal of Behavioral Medicine and Brain Science 2011;20(4):298-301
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of NGF gene modified umbilical cord mesenchymal stem cells (UCMSC) transplantation on neurological functional improvement in traumatic brain rats. Methods Cerebral contusion model in motor-sensory cortex in rats was established by a weight hammer falling method. UCMSC were culutred and transferred with NGF gene. After NGF expression and activity was identified,the NGF gene modified UCMSC were engrafted into injured brain. The neurological function was evaluated 2 weeks after brain injury. And the NGF immunostaining was also performed to explore the level of NGF expression. Results Severe neurological dysfunction( 10.50 ± 0.53 )occurred in rats after traumatic brain injury, while the UCMSC transplantaion led to a significant functional improvement( 7.75 ± 0. 71 )(P ＜ 0. 01 ). Moreover, the best functional improvement was found in rats receiveing UCMSC grafts modified with NGF gene (5.38 ± 0. 52 ) (P ＜ 0.01 ). Conclusion NGF gene modified UCMSC transplantation can improve neural behavior in rats with brain trauma.