Effects of an autophagy/lysosomal pathway induced by 6-hydroxydopamine in PC12 cells

VernacularTitle:6-羟基多巴胺致PC12细胞损伤机制中自噬的影响
Author: Shengkui ZHOU ; Yanbo CHENG ; Runtong GENG ; Hao CHEN ; Han LIU ; Xingshun XU ; Deqin GENG
Publication Type:Journal Article
Keywords: 6-Hydroxydopamine; PC12 cells; Parkinson's disease; Autophagy-lysosomal pathway
From: Chinese Journal of Behavioral Medicine and Brain Science 2011;20(4):312-314
CountryChina
Language:Chinese
Abstract: Objective To investigated the role of the autophagy lysosomal pathway in PD cells and the possible molecular mechanisms. Methods A dopaminergic neuronal injury model was induced by 6-OHDA in PC12 cells . Autophagosomes in PC12 cells were examined by transmission electronmicro-scopy( TEM ). The expression of LC3- Ⅱ , Cathepsin B were assayed by western blot analysis. Results TEM revealed that the autophagosomes were increased in PC12 cells after 6-OHDA treatment and appeared apoptosis. The LC3-Ⅱ (2h:52.57 ±2.27,4h:56.83 ±3.51,6h:73.43 ±5.41,12h:103.90 ±2.57,24h: 100.40 ±3.91 )and Cathepsin B expression ( model group: 113.80 ± 4.46; normal group 35.89 ± 3.40) were increased after 6-OH DA treatments (P ＜ 0.05 or P ＜ 0.01 ). Conclusion The results indicate that autophagy lysosome pathway is involved in 6-OHDA-induced cell death in PC12 cells.