Purified protein derivatives tuberculin from virulent or attenuated strains of Mycobacterium tuberculosis induces cell death of human macrophages in different manner
10.3760/cma.j.issn.1000-6680.2011.01.002
- VernacularTitle:不同毒力结核杆菌的纯化蛋白质衍生物对人巨噬细胞凋亡的诱导及其与Toll样受体-2的相关性
- Author:
Huilian SHI
;
Shenglei YU
;
Chanyi LU
;
Wenhong ZHANG
;
Xinhua WENG
;
Shu CHEN
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Proteins;
Apoptosis;
Toll-like receptor 2;
Macrophages
- From:
Chinese Journal of Infectious Diseases
2011;29(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the cell death in macrophages (THP-1) stimulated with different agonists (H37Rv-PPD or BCG-PPD) and to investigate the relationship between Toll like receptor (TLR)-2 and THP-1 apoptosis. Methods H37Rv-PPD and BCG-PPD were used to stimulate THP-1 cells for 3 h, 8 h, 15 h and 24 h, respectively with or without TLR-2 blockade. Cells were analyzed by flow cytometry to detect the TLR-2 expression. Annexin V staining and Hochest staining were performed to evaluate apoptosis. Results The apoptosis cells were increased when stimulated with BCG-PPD and the percentage was 30.2% at 24 h, which were confirmed by Hochest staining.However, the expression of TLR-2 did not increase simultaneously with percentage of 8.8% at 24 h.Nevertheless, most cells presented with necrosis form when stimulated with H37Rv-PPD and the expression of TLR-2 remained at high level with the percentage of 17.2% at 24 h, while the percent of apoptosis rate was only 7.7%. Under treatment of TLR-2 antibodies, the percentage of apoptosis decreased to 10.5% at 24 h of BCG-PPD stimulation and TLR-2 expressions were down-regulated to less than 3% at all time points; but after H37Rv-PPD stimulation, the percentage of apoptosis and TLR-2 expression did not changed obviously. Conclusions The attenuated BCG-PPD induces THP-1 apoptosis predominately, which is partially correlated with TLR-2 expression. While virulent H37Rv-PPD induces THP-1 necrosis predominately.
