The correlation between APOBEC3G mRNA in peripheral blood mononuclear cells and serum hepatitis C viral RNA level
10.3760/cma.j.issn.1000-6680.2011.02.008
- VernacularTitle:慢性丙型肝炎患者外周血单个核细胞中APOBEC3G mRNA与血丙型肝炎病毒RNA的相关性
- Author:
Jingmin NIE
;
Weiping CAI
;
Fengyu HU
;
Linghua LI
;
Jinfeng LIU
;
Min XU
;
Yujuan GUAN
;
Xiaoping TANG
- Publication Type:Journal Article
- Keywords:
Hepatitis C,chronic;
Nucleoside deaminases;
Monocytes;
Viral load;
RNA messenger;
RNA,viral
- From:
Chinese Journal of Infectious Diseases
2011;29(2):104-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between APOBEC3G mRNA level in peripheral blood mononuclear cells (PBMC) and serum hepatitis C viral RNA level in patients with chronic hepatitis C infection. Methods TaqMan real-time fluorescence relative quantitative polymerase chain reaction (RT-PCR) was used to quantify APOBEC3G mRNA levels in PBMC from 49 patients with chronic hepatitis C (CHC) and 31 healthy subjects. The relationship between APOBEC3G mRNA level and hepatitis C virus (HCV) viral load was analyzed. SPSS11. 0 statistics software was used for t test and regression analysis. Results APOBEC3G mRNA level in CHC patients [(1.5×10-5±1.9×10-5 ) copy/mL] was significantly lower than that [( 5. 2 × 10-5 ± 5. 5 × 10-5 ) copy/mL] in the healthy control subjects (t=-3.005, P＜0.01). While APOBEC3G mRNA level was not related with HCV viral loads (r=-0.082, P＞0.05). Conclusion HCV has an inhibitive effect on APOBEC3G expression, whereas APOBEC3G doesn't affect HCV replication directly in vivo.
