The expression and clinical significance of NKp44+NK cells in the peripheral blood and synovial fluid of patients with rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2011.03.005
- VernacularTitle:类风湿关节炎患者外周血及滑液NKp44+自然杀伤细胞的表达和临床意义探讨
- Author:
Jie REN
;
Zhitao FENG
;
Zhuo LV
;
Juan LI
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Killer cells,natural;
Rheumatoid factor
- From:
Chinese Journal of Rheumatology
2011;15(3):159-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical significance of NKp44+NK cells in the peripheral blood and synovial fluid of rheumatoid arthritis (RA) patients.Methods The proportions of NKp44+NK cells in the PB of 20 active and 15 remission patients with RA and 20 healthy individuals were detected using flow cytometry.The proportions of NKp44+NK cells in the SF of 10 active patients were detected.Multiple nonparametric test was used to compared the difference of NKp44+NK cells proportions.Clinical data including DAS28,anti-CCP antibody and RF were collected.The relationship between NKp44+NK cells and clinical data was analyzed by Spearman correlation analysis.Results The proportions of NKp44+NK cells in PB of active and remission patients and healthy individuals were (1.480±4.750)%,(0.540±0.590)%,(0.000±0.000)%respectively.The proportion of NKp44+NK cells in PB of active patients was significantly higher than that of remission patients and healthy individuals (x2=46.708,P=0.000).The proportion of NKp44+NK cells in the SF of ten active RA patients was significantly higher than matched PB.There was positive correlation between NKp44+NK cells proportion in PB and SF of active patients and DAS 28 and anti-CCP antibody level.There was no correlation between NKp44+NK cells proportion in PB,SF and RF.Conclusion There is a marked increase in the proportion of NKp44+NK cells in PB and SF of RA patients.Moreover,NKp44+NK cells are positively correlated with DAS28 and anti-CCP antibody,suggesting that NKp44+NK cells may be correlated with disease activity and severity.
