Effect of FK506 on expression of axon guidance cue slit-2 after spinal cord injury in rats

VernacularTitle:FK506对大鼠脊髓损伤后轴突导向因子slit-2表达的影响
Author: Wenge LIU ; Kaifu LI ; Zhenyu WANG ; Zhipeng YAO
Publication Type:Journal Article
Keywords: Spinal cord injuries; Nene growth factas; tacrolimus binding proteins; FK506
From: Chinese Journal of Trauma 2011;27(2):166-169
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of FK506 on the expression of axon guidance cue slit-2 after spinal cord injury(SCI)in rats.Methods A total of 75 adult Sprague-Dawley rats were randomly divided into three groups,ie,sham operation group,SCI group and FK506 treatment group.The SCI model was made by using the modified Allen's technique.Then,the rats were sacrificed and the spinal cord was removed at different time points(at days 1,3,7,14 and 28)for detection of the expression of slit-2 by means of reverse transcription polymerase chain reaction(RT-PCR)and immunohistochemistry.Results The expression of slit-2 changed with time.The expression of slit-2 could be detected at day 1 after SCI,reached the highest at day 7 and then decreased gradually,with higher expression level in the injury group compared with treatment group(P ＜ 0.05).Conclusion Following spinal cord injury,administration of FK506 can up-regulate the expression of slit-2 and may exert important effect on the guidance of the axon regeneration.