Influence of PPAR -γligand on radiation - induced PAI - 1 and TGF -βmRNA in fibroblast cells
10.3760/cma.j.issn.1004-4221.2011.02.023
- VernacularTitle:PPAR-γ对放射诱导成纤维细胞分泌PAI-1和TGF-β的影响
- Author:
Yisi ZHANG
;
Daxin ZHANG
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferator activated receptor-γligand;
Plasminogen activator inhibitor-1 gene;
Transforming growth factor-βgene;
L929 cell;
Irradiation
- From:
Chinese Journal of Radiation Oncology
2011;20(2):168-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of peroxisome proliferator activated receptor-γligand (PPAR-γ, pioglatazone) on expression of PAI-1 and TGF-β mRNA and proliferation in fibroblast cells before and after X-ray radiation, and to study the effect of PPAR-γon normal cells during radiation induced fibrosis process. Methods RT-PCR method was used to measure PPAR-γgene expression in L929 cells.After X-ray irradiation of 10 Gy,4 Gy or 2 Gy, the expressions of PAI-1 and TGF-β mRNA in mouse lung fibroblast cells (L929) were measured using RT-PCR. After X-ray irradiation and pioglatazone treatment,the influence of pioglatazone on PAI-1 and TGF-β was measured using RT-PCR method. MTT method was used to test cell proliferation after the treatment of irradiation and pioglatazone. Results PPAR-γ mRNA expression was observed in L929 cells. Expression of PAI-1 and TGF-β mRNA reached the highest level 483.40,P =0. 090) ). At 48 h after the treatment of pioglatazone and 10 Gy radiation, pioglatazone decreased 0. 36, 0. 34 and 0. 32( F = 3.90, P = 0. 040) ). The inhibitory effect was significantly increased when L9292. 50,P =0. 005)). Conclusions X-ray irradiation can increase the expression of PAI-1 and TGF-β in L929 cells. Pioglatazone can decrease the expression of radiation-induced PAI-1 and TGF-β, and restrain the fibroblast proliferation.
