Differential expression profiles of microRNAs in liver of 60 Co γ-ray irradiated mice
10.3760/cma.j.issn.0254-5098.2011.01.004
- VernacularTitle:60Coγ射线照射致小鼠肝脏的miRNAs表达改变
- Author:
Xiujin SUN
;
Fengmei CUI
;
Chengcheng HUANG
;
Mingjiang HU
;
Daojin WANG
;
Yu TU
- Publication Type:Journal Article
- Keywords:
γ-rays;
Irradiation;
miRNA;
Microarray;
Bioinformatic analysis
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differential expression profiles of microRNAs in the liver of 60Co γ-ray irradiated mice using microRNA microarray and to explore their main functions by bioinformatic analysis.Methods After SPF C57BL/6J mice expose to 4 Gy-single whole body radiation,total number of peripheral WBC and the fMNPCE were measured at 3 d.The differentially expressed miRNAs in mouse liver were detected with miRNA microarray,miRNA-124 and miR-34a were confirmed by real time RT-PCR assay.Bioinformatic analysis was applied to explore target genes and the main functions of the differential expressed miRNAs.Results Compared with control group,the total number of peripheral WBC decreased( t = 2.87,P ＜ 0.05 ) ,while the fMNPCE in bone marrow increased ( t =-2.91,P ＜0.05) after 4 Gy γ-ray irradiation.miRNA microarray revealed that 17 miRNAs were differentially expressed,in which 9 up-regulated,8 down-regulated.The expression levels of miR-124 and miR-34a were coincident with the result of real time RT-PCR.GO analysis showed that some pathways including adherens junction and cell cycle were suppressed,while some immune-related pathways were activated.Conclusions miR-34a and miR-194 were involved in the regulation of acute radiation damage,some other miRNAs including miR-124、miR-382 and miR-92a* also played important roles in radiation process.
