Effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro

VernacularTitle:不同剂量60Co γ射线部分照射人离体血对染色体畸变形成的影响
Author: Jianxiang LIU ; Jianlei RUAN ; Mei TIAN ; Yan PAN ; Xu SU
Publication Type:Journal Article
Keywords: Partial irradiation; Human peripheral blood; Chromosome aberration
From: Chinese Journal of Radiological Medicine and Protection 2011;31(1):38-40
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro.Methods The samples of heparinized peripheral whole blood from 3 healthy persons were exposed to 60Co γ-rays at the doses between 0 and 8 Gy with the dose rate of 0.35 Gy/min at the temperature of 37 ℃ ,and then mixed with the unirradiated blood samples of the Microscopy was used to observe the chromosome aberration double ( centromere + centromere) and the biological dose was estimated thereby.ResultsThe amounts of double centromere + centromere were increased along with the dose of irradiation in all groups.The estimated biological dose was higher than the 1/3 of the irradiation dose when the dose was between 0.5 to 2 Gy,and was close to the 1/3 of the irradiation dose when the dose was between 4 to 8 Gy.Conclusion Chromosome aberration can be used as a biomarker in estimation of uneven irradiation.