Activation of NF-κB-HIF-1α pathway induced by X-ray irradiation in human lymphoma cells

VernacularTitle:电离辐射诱导NF-κB-HIF-1α通路在人淋巴瘤细胞中的活化
Author: Qiao QIAO ; Xinyu ZHAO ; Guang LI
Publication Type:Journal Article
Keywords: Lymphoma; NF-κB; HIF-1α; Ionizing radiation
From: Chinese Journal of Radiological Medicine and Protection 2011;31(1):25-28
CountryChina
Language:Chinese
Abstract: Objective To observe the effect of X-ray radiation on NF-κB-HIF-1α pathway activation in three malignant lymphoma and to explore the mechanism of radioresistance mediated by this pathway.Methods Expression levels of p-P65,IKK and HIF-1α in Namalwa,Ramos and Raji cells were determined by Western blot 1,4,10 and 20 h after 5 Gy X-ray irradiation.The effect of QNZ on HIF-1α expression was also observed.Results Expression level of IKK protein was up-regulated 4 h after irradiation in the 3 lymphoma cell strains( t = 8.01,5.14,5.42,P ＜ 0.01 ),followed by up-regulation of p-P65 protein expression level at 10 h and HIF-1α at 10-20 h after X-ray irradiation (t = 11.25,17.43,22.09,P ＜ 0.01 ).Pretreatment of QNZ 24 h before X-ray irradiation significantly reduced the upregulation of HIF-1α protein expression induced by simple ion radiation ( t = 18.69,19.35,12.26,P ＜0.01 ).Conclusion NF-κB - HIF-1α pathway was activated in human lymphoma cells after ion radiation and may be involved in the mechanism of radioresistance.