Expression of chemokine CXCL16 in murine collagen-induced arthritis and the effects on the proliferation of lymphocytes
10.3760/cma.j.issn.1007-7480.2011.03.003
- VernacularTitle:CXCL16在胶原诱导性关节炎小鼠中的表达及对淋巴细胞增殖活化的影响
- Author:
Lin SUN
;
Xiangyuan LIU
;
Jinxia ZHAO
;
Siliang MAN
;
Xia ZHANG
- Publication Type:Journal Article
- Keywords:
Chemokine;
Arthritis,experimental;
Lymphocytes
- From:
Chinese Journal of Rheumatology
2011;15(3):151-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CXCL16 on the development of murine collageninduced arthritis (CIA). Methods CXCL16 mRNA of the involved synovium and serum CXCL16 protein were determined respectively by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA) in murine collagen-induced arthritis. The proliferation of lymphocytes from murine spleen and the level of RANKL mRNA, stimulated by CXCL16 at different concentrations (0,100, 200, 400, 800 ng/ml), was detected respectively by CCK-8 and RT-PCR, then the level of IL-2 and IFN-γ in culture supernatant was detected by ELISA. Comparisons between groups were tested by t test and one-way ANOVA analysis. Results The serum CXCL16 [(127± 10) vs (72±8) pg/ml, P＜0.05] and synovial CXCL16 mRNA (0.214±0.007 vs 0.375±0.009, P＜0.01) in CIA were all significantly higher than those in normal controls. The proliferation of CXCL16 (200, 400, 800 ng/ml) in CIA mouse lymphocytes, was significantly higher than that of CXCL16 (0 ng/ml) (0.51±0.06, 0.56±0.05, 0.55±0.04, (0.41±0.04, P＜0.05). And CXCL16 on the CIA stimulated lymphocyte proliferation was significantly higher than controls on normal lymphocytes (P＜0.05). Compared with blank control group, the expression of IL-2, IFN-γ and RANKL mRNA of CIA CXCL16 (400, 800 ng/ml) groups was higher significantly (P＜0.01). Conclusion CXCL16 plays an important role in the development of murine CIA by activating lymphocytes.
