The expression of Th17 cell in peripheral blood of children with systemic lupus erythematosus
10.3760/cma.j.issn.1007-7480.2011.04.005
- VernacularTitle:系统性红斑狼疮患儿外周血辅助性T17细胞表达的初步研究
- Author:
Leying HUO
;
Huasong ZENG
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
T-lymphocytes,helper-inducer;
Interleukin-21
- From:
Chinese Journal of Rheumatology
2011;15(4):234-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the Th17 cell expression in peripheral blood of childron with systemic lupus erythematosus (SLE) and explore the role of Th17 cells and the cytokines in the pathogenesis of SLE. Methods Twenty-five children with SLE were enrolled and 15 healthy children were controls. Flow cytometry (FCM) was employed to detect the expression of Th17 cells in peripheral blood of SLE children (SLE group, n=25), and IL-17, IL-21 levels in plasma were detected by ELISA. Two-independent sample t-test and Spearmen's test were used for correlation analysis. Results Compared with that of the control, the frequencies of CD3+CD8-IL-17+T[(1.24±0.64)% vs (0.59±0.21)%], CD3+CD8-IL-21+T cells[(1.5±0.6)%vs (0.8±0.4)% ] increased significantly in SLE patients (P＜0.01) and the plasma concentrations of IL-17, IL-21 were significantly higher (P＜0.01). The SLE activity was positively correlated with the frequencies of CD3+CD8-IL-17+T cells, but not with CD3+CD8-IL-21+T cells. Conclusion Th17 cells and the related cytokinesplay an important role in the pathogenesis of childhood SLE.
