Immunological traits of mesenchymal stem cell derived from Umbilical cord Wharton's jelly
10.3760/cma.j.issn.0253-2352.2011.04.010
- VernacularTitle:人脐带Wharton胶间质干细胞的免疫特性研究
- Author:
Shuyun LIU
;
Shibi LU
;
Mei YUAN
;
Li ZHANG
;
Kedong HOU
;
Xifu ZHENG
;
Bin ZHAO
;
Xiang SUI
;
Wenjing XU
;
Quanyi GUO
- Publication Type:Journal Article
- Keywords:
Umbilical cord;
Mesenchymal stem cells;
Immune tolerance
- From:
Chinese Journal of Orthopaedics
2011;31(4):343-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the immunological traits of mesenchymal stem cells derived from umbilical cord Wharton's jelly (WJMSCs). Methods The diced Wharton's jelly which was from healthy fullterm birth human umbilical cord was cultured. The mesenchymal stem cells were identified with mesenchymal stem cells markers expression by flow cytometry and multiple differentiation ability. The expression of MHC- Ⅰ / Ⅱ, costimulatory molecules (CD40, CD80 and CD86) was detected with flow cytomctry, immunocytochemistry, and RT-PCR. The expression of immune inhibitors like HLA-G, IDO, and PGE2 was detected by immunocytochemistry and RT-PCR. The expression of immune-related molecules as IL-10, TGF-β, FGF and VEGF was detected with antibody microarray and western blot. Further more, to clarify the in vivo immune reaction of hWJMSCs, we fabricated the hWJMSC-scaffold constructs and implanted them into the rabbit backs. The lymphocyte infiltration and implanted cell survival observed with immunofluorescence. Results After culturinge of diced Wharton's jelly tissue, we obtained spindle-shaped cells. With differentiation medium, the cells can differentiate into osteoblasts, chongdrocytes, adipose cells and schwann cells. Expression of MHC, costimulatory molecules, and a series of immune suppressive-related molecules was found. Immune inhibitors as HLA-G, 1DO, PGE2, and immune suppressive related molecules as HGF, VEGF, TGFand IL-10 were positively expressed. But the cells did not express MHC-Ⅱ. No immune rejection was observed in vivo after implantation of hWJMSC-scaffold constructs. Conclusion It can be concluded that hWJMSCs have very low immunogenicity, which means the cells have potential to induce immune tolerance.The hWJMSCs do not provoke immune rejection in vivo.
